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. 2021 Mar 19;12:1792. doi: 10.1038/s41467-021-21814-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Peanut agglutinin (PNA) and Galanthus nivalis Agglutinin (GNA) lectin binding to normal hemoglobin (HbAA) RBCs with or without oxidation. Mannan blockade for GNA lectin binding shown in blue. 2 tailed Wilcoxon, paired data, PNA, n = 7; GNA, n = 8 biologically independent RBC donors over two independent experiments. b Immunofluorescence microscopy of GNA lectin/streptavidin (red) staining of healthy HbAA RBCs (above) and after the oxidative insult (below). c Normalized geometric mean fluorescence (gMFI) for binding analyzed by flow cytometry of murine Fc fusions with C-type lectins or sub-domains applied to oxidized versus undamaged RBCs. Mannan blockade of mannose receptor-carbohydrate recognition domain (MR-CRD) binding is shown in blue. MAH, macrophage antigen H. MR-CR, mannose receptor cysteine-rich domain. 2 tailed Wilcoxon, paired data, n = 7 biologically independent RBC donors over two independent experiments. d Immunofluorescence microscopy image of human monocyte-derived macrophages (HMDM) stained with DAPI (blue) and for mannose receptor (green) after incubation with oxidized HbAA RBCs, shown in magenta. e Percentage phagocytosis of oxidized RBCs by HMDM treated with human MR specific or scrambled siRNA. 2 tailed Mann–Whitney, n = 4 biologically independent RBC donors over two independent experiments. f Percentage phagocytosis of healthy or oxidized HbAA RBCs by HMDM with or without pre-blocking by mannan, chitin, or laminarin, 2 tailed Mann–Whitney, n = 4–8 biologically independent RBC donors over two independent experiments. No adjustments made for multiple comparisons. g As f but oxidized RBCs are blocked by MR-CRD blocking antibody 15.2 as indicated. 2 tailed Mann–Whitney, n = 6 biologically independent RBC donors over two independent experiments. h Percentage phagocytosis of sickle cell homozygote (HbSS) RBCs with or without pre-blocking by MR-CRD blocking antibody 15.2. 2 tailed Mann–Whitney, n = 5 biologically independent RBC donors over three independent experiments. i HbAA unblocked and HbSS unblocked or mannan and chitin blockade phagocytosis experiments as shown. 2 tailed Mann–Whitney, n = 4-7 biologically independent RBC donors. No adjustments made for multiple comparisons. All data are shown as median +/− IQR.