Table 3.
Camera | Analysis | Laser wavelength (nm) | Time per frame (ms)a | No. of frames | Image pixel size (nm) | Map dimensions (Pixels) |
---|---|---|---|---|---|---|
EMCCD | FCS | 488 | 2 | 50,000 | 240 | 46 × 46 |
Fig. 2 | SRRF | 488 | 200 | 500 | 24 | 460 × 460b |
EMCCD | FCS | 561 | 2 | 50,000 | 240 | 120 × 56 |
Fig. 4 | Diffusion law | 561 | 2 | 50,000 | 240 | 114 × 56 |
N&B | 561 | 20 | 5,000 | 240 | 120 × 56 | |
SRRF | 488 | 200 | 500 | 48 | 600 × 280c |
aAcquisition time was 2 ms; longer times are reached by time binning of frames.
b,cThe original image has a pixel size of 120 nm (b) and 240 nm (c) at 1 × 1 binning, respectively. During the SRRF analysis, virtual sub-pixels of sizes 24 nm (b) and 48 nm (c), respectively, are created.