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. 2021 Mar 19;12:1748. doi: 10.1038/s41467-021-22002-9

Table 3.

Acquisition parameters for the various experimental configurations.

Camera Analysis Laser wavelength (nm) Time per frame (ms)a No. of frames Image pixel size (nm) Map dimensions (Pixels)
EMCCD FCS 488 2 50,000 240 46 × 46
Fig. 2 SRRF 488 200 500 24 460 × 460b
EMCCD FCS 561 2 50,000 240 120 × 56
Fig. 4 Diffusion law 561 2 50,000 240 114 × 56
N&B 561 20 5,000 240 120 × 56
SRRF 488 200 500 48 600 × 280c

aAcquisition time was 2 ms; longer times are reached by time binning of frames.

b,cThe original image has a pixel size of 120 nm (b) and 240 nm (c) at 1 × 1 binning, respectively. During the SRRF analysis, virtual sub-pixels of sizes 24 nm (b) and 48 nm (c), respectively, are created.