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. 2021 Mar 19;7:28. doi: 10.1038/s41522-021-00193-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Experimental timeline. Germ-free (GF) mice (n = 17) underwent a fecal microbiota transplantation (FMT) of either Dom-derived microbiota (GF/Dom, n = 7), Sub-derived microbiota (GF/Sub, n = 7), or PBS (GF/Con, n = 3). After an adaptation period, the mice underwent a series of behavioral assessments (FST; forced swim test; TCST: three-chamber Sociability test; DSR: dominant–submissive relationship test) and their adipose tissues were removed and analyzed. b Principal component analysis of the gut microbiota of GF/Con, GF/Dom, and GF/Sub mice, (n = 17) at Day 7 post-FMT, demonstrating the clustering of the gut microbiome in each group, as well as in GF mice and in the donor mice. c A heatmap of the microbial composition of the samples at the species level, with the top 100 most variant species identified. Taxa with similar distributions are grouped together. d Relative abundance up to the genus level. An additional figure up to the order level (for significantly altered bacteria) is presented in Supplementary Fig. 4. e LEfSe cladogram of gut microbiota in the different mouse groups. Taxa whose distributions among different groups are significantly different (p < 0.05 and the effect size >2). f Abundance histograms of the Mycoplasmataceae biomarker in Sub mice, detected by LEfSe as the marker of GF/Sub mice. Each bar represents the relative abundance of the specified taxa in an individual mouse. Statistical significance was assessed using a one-way ANOVA with Bonferroni correction.

Fig. 5 — a Experimental timeline. Germ-free (GF) mice (n = 17) underwent a fecal microbiota transplantation (FMT) of either Dom-derived microbiota (GF/Dom, n = 7), Sub-derived microbiota (GF/Sub, n = 7), or PBS (GF/Con, n = 3). After an adaptation period, the mice underwent a series of behavioral assessments (FST; forced swim test; TCST: three-chamber Sociability test; DSR: dominant–submissive relationship test) and their adipose tissues were removed and analyzed. b Principal component analysis of the gut microbiota of GF/Con, GF/Dom, and GF/Sub mice, (n = 17) at Day 7 post-FMT, demonstrating the clustering of the gut microbiome in each group, as well as in GF mice and in the donor mice. c A heatmap of the microbial composition of the samples at the species level, with the top 100 most variant species identified. Taxa with similar distributions are grouped together. d Relative abundance up to the genus level. An additional figure up to the order level (for significantly altered bacteria) is presented in Supplementary Fig. 4. e LEfSe cladogram of gut microbiota in the different mouse groups. Taxa whose distributions among different groups are significantly different (p < 0.05 and the effect size >2). f Abundance histograms of the Mycoplasmataceae biomarker in Sub mice, detected by LEfSe as the marker of GF/Sub mice. Each bar represents the relative abundance of the specified taxa in an individual mouse. Statistical significance was assessed using a one-way ANOVA with Bonferroni correction.