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. 2021 Mar 19;7:28. doi: 10.1038/s41522-021-00193-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Body weight follow-up of transplanted GF mice. The curves represent the average mouse weight gain as a percentage from their weight at the day of transplantation. b–d Mouse social behavior, measured in the TCST. c, d The average velocity (c) and distance (d) per group shows similar locomotor activity in all the groups. e Depressive-like behavior, reflected in immobility time in the FST, in GF/Sub mice, as compared with normal behavior in GF/Dom mice. f Quantification of eWAT mass, obtained from GF mice, normalized to the respective body weight of each mouse. g Histological (H&E) staining of eWAT from GF-transplanted mice (×40 magnification, scale bar = 20 μm). h The eWAT cell diameter of transplanted GF mice was determined using the ImageJ software. Ten random cells per field were analyzed from 10 random fields in each mouse. i An adipokine array comparison of pooled proteins extracted from the eWAT of GF/Sub (n = 4) and GF/Dom (n = 4). j The eWAT adipokines that their expression was significantly altered following FMT. Each bar represents the average of duplicate adipokine expression normalized to the positive control. GF/Sub mice demonstrated a significantly higher adipokine level than GF/Dom mice. Statistical significance was determined using Student’s t test or one-/two-way ANOVA with Bonferroni correction, *p < 0.05, **p < 0.01. Error bars show standard deviation.

Fig. 6 — a Body weight follow-up of transplanted GF mice. The curves represent the average mouse weight gain as a percentage from their weight at the day of transplantation. b–d Mouse social behavior, measured in the TCST. c, d The average velocity (c) and distance (d) per group shows similar locomotor activity in all the groups. e Depressive-like behavior, reflected in immobility time in the FST, in GF/Sub mice, as compared with normal behavior in GF/Dom mice. f Quantification of eWAT mass, obtained from GF mice, normalized to the respective body weight of each mouse. g Histological (H&E) staining of eWAT from GF-transplanted mice (×40 magnification, scale bar = 20 μm). h The eWAT cell diameter of transplanted GF mice was determined using the ImageJ software. Ten random cells per field were analyzed from 10 random fields in each mouse. i An adipokine array comparison of pooled proteins extracted from the eWAT of GF/Sub (n = 4) and GF/Dom (n = 4). j The eWAT adipokines that their expression was significantly altered following FMT. Each bar represents the average of duplicate adipokine expression normalized to the positive control. GF/Sub mice demonstrated a significantly higher adipokine level than GF/Dom mice. Statistical significance was determined using Student’s t test or one-/two-way ANOVA with Bonferroni correction, *p < 0.05, **p < 0.01. Error bars show standard deviation.