Skip to main content
. 2021 Mar 19;4:369. doi: 10.1038/s42003-021-01895-8

Fig. 2. ANAC gene expression at 1 to 7 days after incision.

Fig. 2

a Schematic model of the sampling region from the incised stem for qRT-PCR analysis of b–d. Stem samples (~5 mm length) were separately harvested from the region including the incision (black) and from the nonincised region located ~50 mm above the incision (white). Arrowheads indicate the incision. b–d Relative gene expression of ANAC071, ANAC096, and ANAC011 was analyzed in flowering stems at 1–7 days after incision (DAI). e–g The relative expression levels of ANAC071, ANAC096, and ANAC011 were analyzed in flowering stems at 3 DAI. pin1 mutants were confirmed by T-DNA insertion with PCR and by the phenotype of the pin-formed flowering stem. Values are normalized by ACT2 expression and represent the means of triplicate experiments ± SD. Sampling time at 0 DAI means stem before incision (harvested from same height as incised region; black box in a). Asterisks indicate statistically significant differences compared with 0 DAI or WT (*P < 0.05, **P < 0.01; Dunnett’s test).