Fig. 4. λmax of 39 microbial rhodopsins in solubilized E. coli membrane observed upon hydroxylamine bleach reaction.
The difference absorption spectra between before and after hydroxylamine bleaching reaction of microbial rhodopsins in solubilized E. coli membrane. The λmax of each rhodopsin was determined by the peak positions of the absorption spectra of the original proteins, and the absorption of retinal oxime produced by the reaction of retinal Schiff base and hydroxylamine was observed as a negative peak at ~360–370 nm.