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. 2021 Mar 19;4:366. doi: 10.1038/s42003-021-01891-y

Fig. 7. Large-scale expression and purification of OlZAC.

Fig. 7

a FSEC profiles of OlZAC, as detected by FSEC-Nb for the optimization of cell culture conditions. b Time course curves of the main peak heights, as detected by FSEC-Nb. HEK293S cells were infected with P2 BacMam virus for OlZAC expression at a 1% or 2% volume. At 16 h after virus addition, cell culture temperatures were maintained at 37 °C or shifted to 30 °C. c Size-exclusion chromatography of OlZAC, as detected by UV absorbance. d FSEC trace of purified OlZAC, as detected by Trp fluorescence. e SDS-PAGE of the purified OlZAC after SEC.