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. 2021 Mar 19;11:6453. doi: 10.1038/s41598-021-85733-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Initial analysis of ER-Arm: R-Arm RNASeq data. PCA plot of individual samples shows good clustering of pre-training samples and individual exercise protocols (A). Analysis of differentially expressed genes identified 611 genes expressed differentially at time 0 min (immediately after the exercise) and 489 genes expressed differentially 90 min after the exercise. Out of these, 119 were common (B). Volcano plots of expression fold changes with relation to p-values for individual analyzed time points. R-Arm protocol was chosen as a baseline (C). Top PantherDB-Gene Ontology pathways identified based on differential expression of genes between ER-Arm and R-Arm exercise protocols at two different time points (0 min, 90 min) (D, E).