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. 2021 Mar 19;12:1720. doi: 10.1038/s41467-021-21961-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative images and quantitation of tube length in a Matrigel tube-formation assay (n = 3 biologically independent values in each group) and apoptosis induced by serum starvation assessed by TUNEL staining (n = 3 biologically independent cells in each group) in PAECs transfected with either GFP or INHBA in the presence or absence of recombinant Follistatin (100 ng/mL). TUNEL-positive apoptotic cells are indicated by arrows. b Representative images and quantitation of a Matrigel tube-formation assay and apoptosis induced by H₂O₂ (200 μM/mL) in PAECs treated with conditioned medium (CM) derived from PAECs transfected with either GFP or INHBA in the presence or absence of recombinant Follistatin (100 ng/mL) (n = 3 biologically independent values in each group). TUNEL-positive apoptotic cells are indicated by arrows. c Representative images and quantitation of a Matrigel tube-formation assay (n = 3 biologically independent values in each group) and apoptosis (n = 3 biologically independent cells in each group) in PAECs treated with vehicle or ActA (20 ng/mL) in the presence or absence of recombinant Follistatin (100 ng/mL). TUNEL-positive apoptotic cells are indicated by arrows. Bars: 200 μm (tube-formation assays); 100 μm (apoptosis assays). ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. Exact P values are shown in the Source data file. Data are presented as the mean ± SEM. One-way ANOVA with Tukey’s post hoc test for multiple comparisons was used to compare the tube lengths and apoptotic cell counts between each group for all the figures.