Skip to main content
. 2021 Mar 19;12:1745. doi: 10.1038/s41467-021-22001-w

Fig. 2. Dietary restriction enriches the persulfidation profiles in liver, kidney, muscle, and brain.

Fig. 2

ad Volcano plots showing differentially abundant persulfidated proteins in liver (a), kidney (b), quadriceps muscle (c), and whole brain (d) from ad libitum (AL; n = 4 mice/group) versus 50% dietary restriction (DR; n = 5 mice/group) fed cystathionine γ-lyase (CGL) wildtype (WT) mice. The log2(Fold Change DR:AL) X-axis displays the average fold change in spectral counts for each identified persulfidated protein while the −log10 Y-axis displays the calculated P value when comparing the individual spectral count values for each identified persulfidated protein in a specific tissue from AL versus DR fed mice via a two-sided Student’s t test. The non-axial red dotted vertical lines highlight the biological significance threshold of + /− twofold change in spectral counts between DR versus AL, while the non-axial red dotted horizontal line with asterisk highlights our statistical significance threshold of P < 0.05. The number of total persulfidated proteins identified in each tissue are given next to the tissue name, while blue (AL enriched) and green (DR enriched) colored dots and text indicate persulfidated proteins reaching both biological- and statistical-thresholds. Gray color dots indicate persulfidated proteins not reaching the criteria for both biological and statistical significance for enrichment under either diet. The percentage of proteins skewed toward DR is provided above the tissue label. See also Supplementary Fig. 2.