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. 2021 Mar 19;12:1745. doi: 10.1038/s41467-021-22001-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Volcano plots showing differentially abundant persulfidated proteins in heart (a) and plasma (b) from AL (n = 4 mice/group) versus DR (n = 5 mice/group) cystathionine γ-lyase (CGL) wildtype (WT) mice. The log₂(Fold Change DR:AL) X-axis displays the average fold change in spectral counts for each identified persulfidated protein while the −log₁₀ Y-axis displays the calculated P value when comparing the individual spectral count values for each identified persulfidated protein in a specific tissue from AL versus DR fed mice with P values obtained from two-sided Student’s t test. The number of total persulfidated proteins identified in each tissue are given next to the tissue name, while blue (AL enriched) and green (DR enriched) colored dots and text indicate persulfidated proteins reaching both biological- and statistical-thresholds. Gray color dots indicate persulfidated proteins not reaching the criteria for both biological and statistical significance for enrichment under either diet. The percentage and direction of proteins skewed toward AL is provided above the tissue label. c Venn diagram of shared and non-shared persulfidated proteins in heart and plasma. d KEGG biological pathway enrichment of the shared 78 proteins found in heart and plasma via g:Profiler analysis. e Overlap of AL enriched persulfidated proteins in heart and plasma. f KEGG biological function and pathway enrichment of AL enriched (blue bars) or DR enriched (green bars) persulfidated proteins in heart. g Venn diagram of shared and unshared persulfidated proteins amongst all 6 tissues. h KEGG biological function and pathway enrichment of the shared 28 proteins found in all six tissues via g:Profiler analysis. The numbers in the bars indicate individual persulfidated proteins identified for that specific pathway. Statistical significance for pathway enrichment plotted as the adjusted −log₁₀ (P value) and was auto-calculated via the g:Profiler g:SCS algorithm for KEGG database that utilizes multiple testing corrections. See also Supplementary Fig. 4.