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. 2021 Mar 19;12:1745. doi: 10.1038/s41467-021-22001-w

Fig. 6. NaHS pretreatment augments H2S production capacity of CGL.

Fig. 6

a, b Detection via the lead acetate/lead sulfide method (a) and quantification via integrated densitometry (b) of CGL-derived H2S production in reaction mixtures containing L-cysteine and pyridoxal phosphate after 2 h incubation at 37 °C. c, d Pretreatment with NaHS prior to incubation at 37 °C dose dependently increases CGL-derived H2S production capacity after correcting for detection of non-enzymatic H2S off-gassed in the corresponding (–)CGL wells (c). Plot of the percent change in H2S production relative to the (+)CGL w/0 µM NaHS control. Error bars are ± SEM. The given individual P values are calculated from two-sided t test without multiple comparison test, while asterisks indicate P < 0.05 calculated from one-way ANOVA with Dunnet’s Multiple Comparison Test. n = 4/reaction condition. Source data are provided as a Source Data file. See also Supplementary Fig. 6.