Table 2.

Minimum cryoprotectant concentrations and vitrifiability scores and maximum freezing point calculated at the end of each loading step in Protocol E–D (located at the center of the cartilage cube where x = 0 mm).

Protocol E–D	Minimum concentrations	Minimum vitrifiability score	Maximum freezing point
Loading step 1	2.47 M EG	84	−6 °C
Loading step 2	3.51 M EG 3.12 M DMSO	171	−33 °C