Fig. 4. Infection by NMI ∆dot/icm does not cause body weight loss or splenomegaly in guinea pigs.

a Body weight change is presented as percentage of body weight altered at any given day compared to day 0 post-infection. b Body weight change at 2 days post maximum temperature is presented as body weight change vs expected weight at the measured time point for each individual animal, with a dotted line at 0 indicating no change. c At euthanasia (14 days post-infection), splenomegaly was quantified and data are presented as geometric mean difference of spleen weight normalized to total body weight. Body weight change and splenomegaly were evaluated based on differences in group means (horizontal bar) with associated confidence intervals. Error bars represent standard deviation. For each reported comparison, p-values were computed at the α = 0.05 level for two-sided two-sample t tests, allowing for unequal variances between groups. No adjustment was made for multiple comparisons due to the sample sizes (n = 4) and the descriptive nature of the study. *p ≤ 0.05, **p ≤ 0.01.