Table 2.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Genotype and/or phenotype | Source |
|---|---|---|
| Strains | ||
| E. coli Stellar cells | F–, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, Φ80∆ lacZΔ M15, Δ (lacZYA - argF) U169, Δ(mrr - hsdRMS - mcrBC), ΔmcrA, λ– | Clontech |
| C. burnetii Nine Mile I | NMI, RSA493, phase I, clone 7 | Davis et al.82 and Seshadri, et al.83 |
| C. burnetii Nine Mile II | NMII, RSA439, phase II, clone 4 | Millar et al.56 |
| C. burnetii Nine Mile Crazy | NMC, RSA514 | Hackstadt et al.28 |
| C. burnetii NMI ∆dot/icm | NMI containing a 32.5 kb dot/icm (cbu1622-1652) deletion | This study |
| Plasmids | ||
| pJC-CAT-loxP | pJB2581 containing cat driven by 1169 P; Cmr | Beare et al.84 |
| pUC19-Kan-loxP-sacB | pUC19 containing a 1169P-Kan-loxP-sacB cassette; Kanr | Beare et al.84 |
| pJC-CAT::1169P-lysCA | 1169P-lysCA cassette cloned into pJC-CAT; Cmr | Beare et al.30 |
| pUC19::1169P-cre | pUC19 containing cre driven by 1169 P; Ampr | Beare et al.84 |
| pJC-CAT-loxP::DotIcm3′flank | 3′ flanking DNA from CBU1622 cloned into pJC-CAT-loxP; Cmr | This study |
| pUC19-Kan-loxP-sacB::DotIcm5′flank | 5′ flanking DNA from CBU1652 cloned into pJC-CAT-loxP; Kanr | This study |