Table 3.
Oligonucleotide primers used in this study.
| Primer | Sequence (5’ to 3’) |
|---|---|
| Primers used to create NMI ∆dot/icm | |
| DotIcm5′-pUC19-Kan-loxP-sacB-F | ACGTTTGCGGCCGCCTGCAGCATCGGCTCAACGACCATCC |
| DotIcm5′-pUC19-Kan-loxP-sacB-R | AGGCCTTGCAGGCCCTGCAGGTTGACCGATATAATCATCGGACATC |
| DotIcm3′-pJC-CAT-loxP-F | ACGAAGTTATGTCGACGGTCAGGGATAGTCCCGCTTC |
| DotIcm3′-pJC-CAT-loxP-R | CGGTACCCGGGGATCCGATACCGATCAGGTGCTCGGG |
| LysCAcass-F | GCTCGCGTCGACCGGTGAGCTCGGTACCCGGGGATCC |
| LysCAcass-R | CATGCGCACCACCGGTGATTAATTAGAGAACCTGTTTGTCGAC |
| Primers used for analysis of NMI ∆dot/icm | |
| CBU1628-F | ATTCGAAAAATTTTCCTAACTC |
| CBU1628-R | CAACCCTTCAATCATCAACTTAAC |
| Dot/Icm-KO-F | TTGCCCAGTGGCATAATAAGCG |
| Dot/Icm-KO-R | CTTCTTAACTAGAATATTTATCGCCCATG |
| PCRcontrol-3′-F | CAATAAAAACTAACCCAGAAAGTAATTC |
| PCRcontrol-3′-R | GCTGCTGCATCCGATTCATC |