Fig. 2. Binding mode of GSH and GSO₃⁻.

The GSH bound at the dimer interface establishes chemical interactions (dashed lines) with residues from αH2, αH3, and αH4 of one monomer and αH1 and αH2 of opposite monomer. a Interaction of GSH (yellow ball and stick) at full occupancy site and b GSH at partial occupancy (green ball and stick) with key active site residues of corresponding monomers. c Superposition of GSH conformations in holo-MGST2 at the active site with GSH bound at full occupancy (opaque) and partial occupancy (translucent). d Superposition of active sites of MGST2-GSO₃⁻ complex (opaque) and holo-MGST2 with GSH at full occupancy (translucent).