Skip to main content
. 2021 Mar 17;109(6):947–956.e5. doi: 10.1016/j.neuron.2021.01.008

Figure 2.

Figure 2

Stress triggers cognitive deficits and reduces AMPAR transmission in LHb neurons

(A) Experimental timeline. Boxplots and scatterplots of the error incidence (control, black: n = 20 mice, 5.8 ± 0.6633 during habituation and 6.05 ± 1.006 during test; stress, red: n = 17 mice, 5.706 ± 0.6847 during habituation and 9.353 ± 0.895 during test). Two-way repeated-measure ANOVA (F1,35 = 6.297) with Sidak correction (∗∗p < 0.01, habituation versus test).

(B) Single-mouse heatmap of LHb fluorescence aligned to entry into the non-rewarded container (error dip) during the test session (scale bar: 2 s).

(C) Time course of the average LHb fluorescence aligned to entry into the non-rewarded container (error dip) during the test session. Boxplots and scatterplots of the AUC (control, black: n = 5 mice/69 trials, 12.549 ± 1.987 during habituation; n = 5 mice/76 trials, 30.331 ± 3.363 during test; stress, red: n = 5 mice/70 trials, 12.276 ± 1.57 during habituation; n = 5 mice/91 trials, 14.841 ± 2.144 during test). Two-way ANOVA (F1,302 = 10.56) with Sidak correction (∗∗∗p < 0.001, control versus stress).

(D) Example traces of AMPA/NMDA (scale bars: 30 pA and 10 ms). Boxplots and scatterplots of AMPA/NMDA (control, black: n = 6 mice/26 cells, 2.105 ± 0.4023; stress, light red: 24 h, n = 4 mice/13 cells, 0.76 ± 0.1717; dark red: 7 days, n = 3 mice/12 cells, 0.9567 ± 0.2753). One-way ANOVA (F2,48 = 4.042) with Holm-Sidak correction (∗p < 0.05, comparisons against control).

(E) Array tomography images obtained from single 100-nm slices immunolabeled against Synapsin (red) and GluA1 (green) (scale bar: 5 μm). White arrows indicate representative puncta showing co-localization of both markers. Boxplots and scatterplots of the number of puncta per cubic micrometer (control, black: n = 4 mice, 1.633 ± 0.0615 for Synapsin, 1.289 ± 0.0319 for GluA1 and 0.769 ± 0.0207 for Synapsin/GluA1; stress, red: n = 4 mice, 1.616 ± 0.1474 for Synapsin, 1.01 ± 0.0723 for GluA1 and 0.5303 ± 0.0282 for Synapsin/GluA1). Unpaired Student’s t test (GluA1: t6 = 3.525, ∗p < 0.05; Synapsin/GluA1: t6 = 6.828, ∗∗∗p < 0.001).

Data are represented with heatmaps, boxplots (median and quartiles), or mean ± SEM. See also Figures S2 and S3.