Figure 3.

Stress-driven depression of habenular AMPAR transmission is not input-specific

(A) Schematic of the experimental design. Representative injection sites of AAV-CoChR (top, scale bar: 200 μm) and the corresponding terminal field within the LHb (bottom, scale bar: 100 μm). LH, lateral hypothalamus; f, fornix; EPN, entopeduncular nucleus; Am, amygdala; CPu, caudate putamen; VP, ventral pallidum; LS, lateral septum; d/vBNST, dorsal/ventral bed nucleus of the stria terminalis; AC, anterior commissure; LPO, lateral preoptic area; SNr/c, substantia nigra pars reticulata/compacta; mVTA, medial ventral tegmental area; IPN, interpeduncular nucleus.

(B) Example traces of input-specific AMPA/NMDA (scale bars: 50 pA and 10 ms for LH, EPN, and BNST; 10 pA and 10 ms for mVTA). Boxplots and scatterplots of AMPA/NMDA (control, black: LH, n = 2 mice/8 cells, 0.9538 ± 0.1864; EPN, n = 5 mice/20 cells, 3.164 ± 0.8938; BNST, n = 4 mice/13 cells, 1.102 ± 0.235; mVTA, n = 3 mice/10 cells, 1.633 ± 0.5646; stress, red: LH, n = 3 mice/9 cells, 0.3944 ± 0.0559; EPN, n = 7 mice/22 cells, 1.431 ± 0.4577; BNST, n = 5 mice/13 cells, 0.5162 ± 0.0799; mVTA, n = 2 mice/8 cells, 0.3113 ± 0.0636). Mann-Whitney test (LH: U = 10; EPN: U = 110; BNST: U = 41.5; mVTA: U = 12.5) (^∗∗p < 0.01, ^∗p < 0.05).

Data are represented with boxplots (median and quartiles) or mean ± SEM. See also Figure S3.