Figure 4.

Particles bearing the S proteins of SARS-CoV-2 variants exhibit similar stability and entry kinetics

(A) Particles bearing the indicated S proteins were incubated for different time intervals at 33°C, snap frozen, thawed, and inoculated onto Vero cells. Entry of particles that were frozen immediately was set as 100%.

(B) Particles bearing the indicated S proteins were incubated for the indicated time intervals with Vero cells. Subsequently, the cells were washed and luciferase activity determined. Transduction measured for particles incubated with cells for 24 h (maximum incubation time = time of luciferase measurement) was set as 100%.

For both panels, the average (mean) data from three biological replicates (each performed with technical quadruplicates) is presented. Error bars indicate the SEM. Statistical significance of differences between WT and variant S proteins was analyzed by two-way ANOVA with Dunnett’s posttest (p > 0.05, not significant [not indicated]; p ≤ 0.05, *; p ≤ 0.01, **; p ≤ 0.001, ***).