Figure 11.
Calculated fluorescence x-t images of simulated CREs. The color-coded contour plots indicate the fluorescence increase of 50 µM Fluo-4 in the cytosol as if recorded in the line-scan mode at position (micrometer) and time (millisecond) from the point source (narrow CRS, NRYR = 20, iCa = 0.25 pA) in response to release fluxes calculated for the average time courses of quarks, blips, and sparks (corresponding to blue traces in Fig. 8 A, left). The white contour lines (middle and right) indicate the detection limit of 0.3 ΔF/F0 units; the quark (left) did not reach the detection limit. The corresponding time and spatial fluorescence profiles at the peak are shown at and above the images. The colormap shown on the right side is common to all three panels.