FIGURE 6.

Anti‐DKK1 increases long‐term persistence of human ASCs within femoral segmental defects. Defects were treated with ASC seeded scaffolds or acellular control scaffolds. Animals were treated with anti‐DKK1 or IgG control (15 mg/kg, SC, twice weekly). A‐C, Immunofluorescence staining of human‐specific nuclei (HuNu) at 8 weeks postimplantation. A,A′, Acellular IgG control; B,B′, IgG + ASCs; C,C′, anti‐DKK1 + ASCs. Human cells appear red while DAPI nuclear counterstain appears blue. D, Quantification of HuNu immunohistochemical detection (% positive cells per random 10x field of view, n = 4 samples per group analyzed). White scale bars = 50 μm. *P < .05. ASCs, adipose‐derived stem cells. DAPI, 4′,6‐diamidino‐2‐phenylindole