Figure 7.

Shared dysregulated genes are linked to neurological disease

(A) Venn diagram showing the overlap between significantly dysregulated genes in KO (6 weeks), MM2-EGFP (6 weeks), and MM2-EGFP (12 weeks), each compared to age-matched WT controls. Significance cutoff: adjusted p (padj) < 0.05.

(B) Shared genes are dysregulated in the same direction in KO/WT (6 weeks) and MM2-EGFP/WT (12 weeks).

(C) Heatmap showing transcriptional changes in the 20 neurological disease-associated genes. The 3 datasets in this study were compared to an independent KO/WT (6 weeks) hypothalamus dataset (Chen et al., 2015) and a KO/WT (8 weeks) cortex dataset (Boxer et al., 2019). Avpr1a is not expressed in cortex (X).

(D) Comparison of mCG + mCAC binding sites between unchanged genes, and dysregulated genes in KO only, MM2-EGFP only, both mutants and disease-associated genes.

(E) Ratios of mCAC to total binding sites in these gene categories.

(F) Expression changes in KO/WT and MM2-EGFP/WT in these gene categories. Black bars show median values; whiskers show 5th–95th percentiles. Pairs of gene sets were compared using Mann-Whitney tests: ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.