Table 1. Protein combinations used to measure kinetics and steady-state tip complex composition with two regulators.
| Actin fluorophore | SNAP TOCA-1 fluorophore | Third protein | Number of time-lapse experiments | Number of time-lapse fields of view | Number of snapshot experiments | Number of snapshot fields of view |
|---|---|---|---|---|---|---|
| Alexa 568 | Alexa 647 | eGFP-Diaph3 | 3 | 9 | 6 | 10 |
| Alexa 568 | Alexa 647 | eGFP-Fascin | 2 | 6 | 5 | 46 |
| Alexa 568 | Alexa 488 | SNAP–Ena Alexa 647 | 3 | 9 | 6 | 43 |
| Alexa 647 | Alexa 488 | mKate-GBD | 3 | 12 | 2 | 26 |
| Alexa 647 | Alexa 488 | KCK–VASP Alexa 568 | 2 | 8 | 3 | 16 |
| Alexa 568 | Alexa 488 | SNAP–N-WASP Alexa 647 | 5 | 17 | 0 | 0 |
eGFP, enhanced GFP.