Fig. 6.

HIF-1α nuclear accumulation in HaCaT cells exposed to Nano-Ni. Expression of HIF-1α in HaCaT cells was determined by Western blot after cells were exposed to 10 or 20 μg/mL of Nano-Ni or Nano-TiO₂ for 24 h (a), or to 20 μg/mL of Nano-Ni for 12, 24, 48, or 72 h (c). The representative results of three Western blot experiments are shown. Cells without treatment were used as control. Equal protein loading was verified by Coomassie Brilliant Blue staining. b & d are normalized band densitometry readings averaged from three independent experiments ± SE. * Significant difference as compared with the control group, p < 0.05