Table 1.
Citation information for cellular localization of proteins previously reported as localizing in mitochondria but not observed in ultrapurified mitochondria here
| Protein | Localization | Tissue (species) | Method | Mitochondria purity | ||
| Main | Mitochondrial | Cell | Organ | |||
| Cav1.2α1 | Plasma membrane | Ref. 23 | HT22 (ms) | Brain (ms) | IB | Semipure |
| Eaat1 | Plasma membrane | Ref. 49 | — | Heart (ms) | IB, IF, fractionation | Crude |
| Mct1 | Plasma membrane | Ref. 41 | Neurons (rt) | Brain (rt) | IF, IB | Crude |
| Nmdar1 | Plasma membrane | Ref. 29 | — | Brain (rt) | IB, EM | Semipure |
| Gapdh | Cytoplasm | Ref. 37 and MitoCarta | HeLa (hu) | Liver (rt), brain (ms) | EM, IB, fractionation | Crude and ultrapure |
| Ryr1 | ER | Ref. 33 | Neurons (rt) | Brain (rt) | IB, IF, fractionation | Crude and semipure |
The main site of cellular localization and the reference(s) for reported mitochondrial localization are specified. Reported reference cell type, species (ms, mouse; rt, rat; gb, gerbil; hu, human), and localization method (IB, immunoblot; IF, immunofluorescence; EM, electron microscopy) are also indicated. Mitochondria purity was approximated to the designations used in this study.