Fig. 3.

Kinase activation upon Zn²⁺ addition. (A) Log₂ fold change in phosphorylation versus Log₂ (P) for a series of human kinases upon Zn²⁺ treatment of HeLa cells. A Proteome Profiler Human Phospho-Kinase Array was used to evaluate changes in phosphorylation for a panel of kinases from cells either untreated or treated with 40 μM Zn²⁺ for 30 min. Two biological replicates were run per condition. The pink box represents Log₂ fold change < −1 and P < 0.05. The green box represents Log₂ fold change > 1 and P < 0.05. Details are in SI Appendix, Table S2. (B) Western blots for total and phosphorylated protein of MEK, ERK, and CREB show activation after treatment with different concentrations of Zn²⁺ for 30 min. Individual antibody blots were run on separate gels, cut at ∼48 kDa to compare with β-actin loading control (Fig. S4), and contrast was adjusted individually using ImageJ. Blots are representative of at least four separate experiments.