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. 2021 Mar 8;118(11):e2012228118. doi: 10.1073/pnas.2012228118

Fig. 1.

Fig. 1.

Overall strategy and technological platform validation. (A) HK2 overexpression in a wide range of cancer cell lines. (B) The working flow of CTC enrichment, identification, and single-cell manipulation. A PDMS microwell chip was used with each well 30 μm in diameter and 20 μm in height. (C, Left) Colocalization of HK2 and MitoTracker Green in H1975 cells. White blood cells (WBC) are used as a negative control for HK2 staining. (C, Right) Fluorescence image of HK2-stained H1975 cells at 60× magnification. HK2 staining exhibits a fragmented morphology, with many spheroid-shaped staining, or a reticulated morphology, very similar to the morphology of mitochondria. (Scale bar: 15 μm.) (D) Violin plots of HK2 intensity of H1975 and HCC827 cells that were normalized to the mean of white blood cells (n = 591, 433, and 1,027, respectively). The dashed and dotted lines of each violin plot denote the median and first and third quartiles, respectively. The gray dashed line indicates the average HK2 intensity of leukocytes plus five SDs.