Fig. 2.

FQ-induced cleavage is inhibited by MsMfpA in an ATP-dependent manner. Effect of MsMspA on (A) CFX- or (B) MFX-induced cleavage complexes with Msgyrase. (A) As a control, cleavage assays with Msgyrase were performed in the absence of FQs and the presence of MsMfpA. The inhibitory effects of MfpA on CFX- (C) or MFX- (D) induced cleavage reactions were abolished in the absence of ATP or in the presence of ADPNP. I: intact DNA; L: linear DNA; N: nicked DNA. Note that the gel is run in the presence of ethidium bromide.