Fig. 5.

MeV-infected cells shed into the apical compartment detected by flow cytometry. TEC cultures were infected with WT MeV or LAMV for 4 h and washed with PBS. (A) Percentage of shed cells that were LAMV-infected determined by intracellular staining for MeV-N and flow cytometry using gates generated based on mock-infected cultures at 24, 72, and 120 h. (B) Percentages of shed cells infected with LAMV or WT MeV after apical (blue) or basolateral (orange) infection. Graph presents results from two independent experiments with duplicates or triplicates for each experiment. (C) Amounts of infectious virus produced by cells and supernatant fluids of the apical compartment after apical and basolateral infection with LAMV. Cells and supernatant fluids before and after separation by centrifugation were assayed with a TCID₅₀ assay. Both cell-associated virus and cell-free virus were found in the apical compartment.