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Workflow of mass cytometry. Antibodies conjugated with varied metal isotopes are first applied to stain the protein targets. Subsequently, cells are nebulized into single-cell droplet, and passed through an inductively couple plasma (ICP) time-of-flight (TOF) mass spectrometer to obtain an elemental mass spectrum for every single cell (Reprinted with permission from Bendall and Nolan (2012). Copyright 2012 Nature Publishing Group)
