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Workflow of single-cell Western blots. The process begins as individual cells are settled and lysed in the microwell, followed with single-cell PAGE, immobilization of proteins onto the gel by UV, and in-gel probing with fluorescent antibodies. Through reiterative cycles of antibody removal and protein relabeling, comprehensive protein analysis can be achieved in single cells (Reprinted with permission from Sinkala et al. (2017). Copyright 2017 Nature Publishing Group)
