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. Author manuscript; available in PMC: 2021 Mar 20.
Published in final edited form as: Wiley Interdiscip Rev Syst Biol Med. 2020 Aug 3;13(1):e1503. doi: 10.1002/wsbm.1503

FIGURE 7.

FIGURE 7

Approaches to erase fluorescence signals in reiterative immunofluorescence. (a) Fluorescence signals are eliminated using photobleaching or chemical bleaching. (b) Stripping solution is applied to elute antibodies from their protein targets. (c) The fluorescent oligonucleotides hybridized to oligonucleotide-conjugated antibodies are removed with DNA strand displacement reactions. (d) In the CO-Detection by indEXing (CODEX) approach, the fluorophores introduced by incorporation of the cleavable fluorescent nucleotide is removed by chemical cleavage of the linker to release the fluorophores from the incorporated nucleotides. (e) The signals generated by cleavable fluorescent antibodies are erased by chemical cleavage of the linker to release the fluorophores from antibodies