Figure 1.

Integrin α10 antibodies bind specifically to the integrin α10 subunit. (A) Binding of the integrin α10 antibodies Th101 and mAbα10 and the human isotype control antibody Th301 to C2C12 cell lines overexpressing integrin α10 (C2C12α10) or integrin α11 (C2C12α11). (B) Specific binding of Th101 and mAbα10 to C2C12α10 cells. The gates were set to unstained cell samples. The figure shows cell plot shift when the C2C12α10 cells were stained with the integrin α10 human Th101 and mouse mAbα10 antibodies, but not with human Th301 and mouse immunogloblin G 2a (IgG2a) control antibodies or integrin α11 mouse monoclonal antibody (mAbα11). In C2C12α11 cells, there was a clear cell plot shift when the cells were stained with mAbα11, but not with Th101 and mAbα10. (C) Binding competition assay between the integrin α10 antibodies mAbα10 and Th101 using C2C12α10 cells. Data are expressed as mean fluorescence intensity (MFI) of 100,000 cells. Cells were incubated with antibodies at the indicated concentrations (μg/mL) and subsequently incubated with secondary antibodies. Immunolabeled cells were analyzed by flow cytometry. Data are compiled from at least three independent experiments as mean ± SE.