Figure 3.

Integrin α10 antibodies inhibit GB cell adhesion to collagen and gelatin. Adhesion of C2C12α10 (A), U3054MG (B), and U3046MG (C) cells to dishes pre-coated with collagen I (Col I) or gelatin for 1 h in the absence or presence of integrin α10 antibodies (Th101 and mAbα10) or control antibodies (Th301 and IgG2a). Bovine serum albumin (BSA)-coated wells were used as a negative control. Adhered cells were quantified by crystal violet and spectrophotometric analysis. The bar graph shows the percentage of cell adhesion compared with non-treated (NT) cells. Data are compiled from three independent experiments as mean ± SE. Unpaired two-tailed Student t-test was applied. Four levels of significance were used for a 95% confidence interval, where * p < 0.05; *** p < 0.001; and **** p < 0.0001.