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. 2021 Mar 19;21:178. doi: 10.1186/s12935-021-01872-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The effect of stable transfection of pre-mir-34a on target genes expression. a Sequence of DNA fragment, 248 base pairs in length, including pre-mir-34a (highlighted in grey) and flanking sequences was obtained from the human gene. The DNA fragment was cloned in the MCS between BglII and HindIII restriction sites. The RNA expression levels of miR-34a-5p, β-catenin and Notch1were quantified using qRT-PCR analysis. b The miR-34a-5p expression was significantly enhanced in KatoIII-pGFPC1-34a cells compared with the equivalent control cells, KatoIII-pGFPC1-empty (p < 0.05). c A non-significant mild reduction of β-catenin mRNA level was determined in KatoIII-pGFP-34a cells relative to the KatoIII-pGFPC1-empty cells. d The Notch1 mRNA level was significantly decreased in KatoIII-pGFP-34a cells relative to KatoIII-pGFPC1-empty cells (p < 0.01). e The expression level of β-catenin protein was detected using western blot analysis. The data demonstrated that the β-catenin protein expression was significantly reduced in KatoIII-pEGFPC1-34a cells relative to the control cells (p < 0.05)