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. 2021 Mar 19;14:46. doi: 10.1186/s13045-021-01059-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — TRAF3IP2-AS1 expression is significantly decreased in NONO-TFE3 tRCC. a The RNA level of TRAF3IP2-AS1 (red) was analyzed by FISH assays in NONO-TFE3 tRCC and ccRCC. DAPI-stained nuclei are blue. b–d Kaplan–Meier analysis revealed the disease-free survival (DFS) in KICH, KIRC and KIRP patients based on the relative TRAF3IP2-AS1 expression. e The RNA level of TRAF3IP2-AS1 was analyzed by qRT-PCR assay in ccRCC cell line (786-O), tRCC cell lines (UOK109 and UOK120) and normal cell lines (HK-2 and HEK293T). f–h Analysis of TRAF3IP2-AS1 in KICH, KIRC and KIRP tissues compared with normal tissues were performed using TCGA data. i–j The subcellular distribution of TRAF3IP2-AS1 was analyzed via qRT-PCR in HEK293T, HK-2, 786-O, UOK120 and UOK109 cells. U6 and GAPDH were used as nuclear and cytoplasmic markers, respectively. k The location of TRAF3IP2-AS1 (red) in UOK109 and 786-O cells was determined by FISH assay. U6 and 18s rRNA were used as positive controls for the nuclear and cytoplasmic fractions, respectively. DAPI-stained nuclei are blue. The data are presented as the mean ± SD, *P < 0.05, ***P < 0.001