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. 2021 Mar 19;14:46. doi: 10.1186/s13045-021-01059-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TRAF3IP2-AS1 down-regulates PARP1 mRNA by direct binding. a The RNA levels of TRAF3IP2-AS1 and PARP1 were detected after transfection with TRAF3IP2-AS1 plasmids or siTRAF3IP2-AS1 for 48 h. b Level of PARP1 mRNA detected by qRT-PCR after MS2-RIP for GFP in UOK109 cells. AS1, NC, AS1-antisense and AS1-Mut correspond to TRAF3IP2-AS1, empty vector, TRAF3IP2-AS1-antisense and TRAF3IP2-AS1 with mutation of potential binding site to PARP1 mRNA, respectively. c–d Dual Luciferase Reporter Assay used to detect the relative luciferase activity in HEK293 T cells co-transfected with siTRAF3IP2-AS1 and pmirGLO-PARP1 3′-UTR WT/MUT. e The stability of PARP1 mRNA and GAPDH mRNA in UOK109 cells transfected with TRAF3IP2-AS1 or TRAF3IP2-AS1 contained binding site mutation was measured by qRT-PCR relative to 0 h after blocking new RNA synthesis with α-amanitin and normalized to 18 s rRNA. f The RNA levels of NONO-TFE3 and PARP1 were detected after transfection with NONO-TFE3 plasmids or shTFE3 for 48 h. g The stability of PARP1 mRNA and GAPDH mRNA in 786-O cells transfected with siTRAF3IP2-AS1 was measured by qRT-PCR after treatment with α-amanitin. h The protein level of PARP1 was detected after transfection with TRAF3IP2-AS1 plasmids or siTRAF3IP2-AS1