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. 2021 Mar 19;21:144. doi: 10.1186/s12870-021-02925-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Genome-wide association study results for SNS and an analysis of the peak on chromosome 7A. a Manhattan plots for SNS. The horizontal line represents the significance threshold. The arrows indicate the location of the main peaks studied. b Genomic locations of four SNP loci and the LD based on paired R² values between SNPs on chromosome 7A. c Haplotypes detected in 543 accessions based on the four SNPs. d Differences in the SNS among three haplotypes. e Structure of the TraesCS7A01G482000 gene. f Transcriptomic analysis of TraesCS7A01G482000 in the spike during different developmental stages. Gene expression was based on the fragments per kilobase of transcript per million mapped reads (FPKM) value