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. 2021 Mar 16;14:1911–1922. doi: 10.2147/OTT.S301412

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© 2021 Jin et al.

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Both intra- and extracellular miR-9 level upregulated in irradiation-induced M1 microglia. (A) Heat map of miRNA microarray data shows differentially expressed miRNA pattern between LPS-treated and PBS-treated microglia. Red indicates relatively up-regulated genes. Green indicates relatively down-regulated genes. The red box in panel A showed that miR-9 was significantly upregulated in LPS-treated M1 microglia. (B) qRT-PCR results of intracellular miR-9 expression in CHME5 cells after 0 Gy, 3 Gy, 3 Gy*2f, irradiation. (C) qRT-PCR results of extracellular miR-9 expression in CHME5 cells after 0 Gy, 3 Gy, 3 Gy*2f, irradiation. (D) qRT-PCR results of intracellular miR-9 expression in HMO6 cells after 0 Gy, 3 Gy, 3 Gy*2f, irradiation. (E) qRT-PCR results of extracellular miR-9 expression in HMO6 cells after 0 Gy, 3 Gy, 3 Gy*2f, irradiation. Data are mean ± SD. ns, P>0.05, *P<0.05, ***P<0.001; ****P<0.0001.