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. 2021 Mar 16;14:1911–1922. doi: 10.2147/OTT.S301412

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© 2021 Jin et al.

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Irradiated M1 type microglia inhibited the MET via miR-9/CDH1 axis. (A and B) Protein expression of E-cadherin and vimentin in A549 cells and A549-F3 cells. All groups of A549 cells transfected with negative control, miR-9 mimics (up+), miR-9 inhibition (down+) plasmid were firstly treated with TGF-β1 (TGF-β1+). Then, controlled culture media, cell culture supernatant of CHME5 cells with or without irradiation (IR+/IR-), were added into those TGF-β1-treated A549 cells or A549-F3 cells, respectively. (C) Schematic representation of the 3ʹ-UTR of CDH1 with the predicted target site for miR-9-5p. The mutant site of CDH1 3ʹ-UTR is indicated as red font (without line). (D) Relative luciferase activity was assayed in different groups. H15279: WT; H15280: MUT; NC: scramble miRNA mimic; miR-9: miR-9-5p mimic. Data are mean ± SD. ***P<0.001, ****P<0.0001, *****P<0.00001.