Figure 6. Increased proliferation of cardiac Sca-1 non-immune cells in control but not in ErbB3^MyeKO male mice.

A. Representative flow cytometry plots demonstrating the percentage of CD31^posSca-1^pos endothelial cells (upper right quadrant) and Sca1^posCD31^neg cardiac fibroblasts (lower right), within CD45 negative (non-immune population) in control (MyeWT, upper panel) and ErbB3 knockout (MyeKO, lower panel) male mice. The population of Sca-1 positive non-immune cells is shown in the blue gate. B-E. Total number of (B) endothelial cells, (C) Sca-1 ^posCD31^neg cells, (D) proliferating Sca-1 positive cells (blue gate), and (E) apoptotic Sca-1 positive cells, defined as a phosphatidylserine (PS) positive cells, within the blue gate in control (WT, open) and myeloid cell-specific ErbB3 knockout (KO, grey-shaded) male mice. Sham - n=7, control; n=7, KO; day 3 after TAC - n=9, control; n=8, KO. F-J. Flow cytometry plots (F) and the total number of (G) endothelial cells, (H) cardiac fibroblasts, (I) proliferating and (J) apoptotic Sca-1 positive cells in female mice. Sham - n=6, control; n=6, KO, and on day 3 post-TAC - n=9, control; n=9, KO. Horizontal lines are mean values. One-way ANOVA, Tukey's multiple comparisons test.