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. Author manuscript; available in PMC: 2022 Apr 10.
Published in final edited form as: Cancer Lett. 2021 Jan 22;503:91–102. doi: 10.1016/j.canlet.2021.01.015

Figure 5. Genetic deletion of MUC4 induces apoptosis in PDAC cells.

Figure 5.

A and B Apoptosis analysis using Annexin V/FITC in T3M4 WT, WT-MUC4KO (3B4 and 4D3), T3M4 SC, and SC-MUC4KO (1E11 and 5D10) cells by FACS (mean ± SD, n=3). C. Western blotting of Mcl-1, Bcl-2, and cytochrome c in T3M4 WT, WT-MUC4KO (3B4 and 4D3), T3M4 SC, SC-MUC4KO (1E11 and 5D10) cells. β-actin acts as a loading control. D. Immunohistochemical analysis of cleaved caspase 3 in T3M4 WT, WT-MUC4KO (4D3), T3M4 SC, and SC-MUC4KO (5D10) cells implanted xenograft tumors. E. Average IHC histoscore of cleaved caspase 3 (n=5). Live/Dead cell analysis of anti-Tn-MUC4 mAb 3B11 (5 μg/ml, for 24 h) treated T3M4 WT and SC cells (F and G), and Capan-2 WT and SC cells (H and I) (mean ± SD, n=3). [ns = not significant].