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. Author manuscript; available in PMC: 2022 Apr 10.
Published in final edited form as: Cancer Lett. 2021 Jan 30;503:163–173. doi: 10.1016/j.canlet.2021.01.024

Figure 3. Differential gene expression between parental and IP3 cell lines.

Figure 3.

From the list of top 20 differentially expressed genes shown in Table 1, qPCR validation was performed to verify differential expression of 10 genes. Total RNAs were isolated from cells, quantified, and reverse transcribed using 1 ug RNA. qRT-PCR was performed using synthesized cDNA (40x dilution), primers (200 nM, Supplemental Table 1) and iTaq Universal SYBR Green Supermix (BioRad, Hercules, CA). qRT-PCR conditions were as specified in Materials and Methods. Relative gene expression was determined with the comparative delta-delta Ct method and normalized using HPRT1 and HSPCB reference genes. Panels depict the expression ratio of IP3 to corresponding parental cell line. Experiments were performed in at least triplicate and results show mean +/− SEM.