Skip to main content
. Author manuscript; available in PMC: 2022 Apr 10.
Published in final edited form as: Cancer Lett. 2021 Jan 30;503:163–173. doi: 10.1016/j.canlet.2021.01.024

Figure 5. AMIGO2 regulates multicellular aggregate dynamics.

Figure 5.

(A) MCAs were generated via the hanging drop method by seeding cells at a density of 300 cells per 20 μl droplet onto the lid of culture dishes. Dishes were gently inverted and allowed to aggregate for 2 days. MCAs were imaged using Echo Revolve hybrid microscopy in bright field and fluorescence modes. Shown are parental OVCAR5 cells, OVCAR5-IP3, OVCAR5-IP3-AMIGO2-KD or OVCAR5-IP plus anti-AMIGO2 antibody as indicated. (B,C) MCA areas (μm2) were measured using ImageJ and statistical analysis was performed using one-way ANOVA. Experiments were performed in triplicate.