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Validation of TraDIS results with single-gene knockouts. (A), Survival curves for G. mellonella larvae following infection with K. pneumoniae RH201207 and mutants in defined genes. (B), Complementation of the virulence defect of RH201207 ∆nfeR. Mutants where survival is significantly different to wild-type (Kaplan–Meier test) are indicated by asterisks (*, P <0.05; **, P < 0.01; ***, P < 0.001).
