Figure 1.
MB ablation alters metabolism in p53172H/H mice. Male mice of the indicated genotypes without evidence of tumor were used for the following experiments. A, Immunoblot of indicated proteins in skeletal muscle of 10 wk old mice. Actinin and VDAC serve as protein loading controls. B, Measurement of body composition relative to body weight (BW) in 10 wk old mice (n = 21). C, Relative levels of some metabolites related to mitochondrial activity identified by metabolomic profiling of skeletal muscle (10 wk old mice, n = 7–8). D, Lactate levels in tail blood of ~12 wk old mice (n = 19). E, Ex vivo oxygen consumption rate (OCR) of whole soleus muscle from aged-matched mice (n = 6). Statistical difference by 2-tailed unpaired t test. Values are mean ± SEM. *P < 0.05.