Figure 3.

Aberrant lipid metabolism and cell signaling in p53^172H/H thymus are corrected by MB ablation. Thymi of male mice (10 wk old) were used for the following experiments. A, Heat map of metabolites identified by metabolomic profiling that were significantly different between MB^+/+ p53^172H/H and MB^−/− p53^172H/H mice (P < 0.05, n = 5). Metabolite ion abundance was normalized to the median and then log2 transformed. Color is based on the normalized ion abundance in the range of -1.0 to 0.6 (blue, lowest abundance; red, highest abundance). B, Effect of MB deletion on the reduced levels of 5’-GMP and 2-oleoylglycerol in p53^172H/H mice (n = 5). C, Immunoblots of thymic tissues from mice of indicated genotypes. *P < 0.05.