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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Cancer Prev Res (Phila). 2020 Sep 21;14(1):31–40. doi: 10.1158/1940-6207.CAPR-20-0368

Figure 4.

Figure 4.

Expression of human R175H mutant p53 (mouse p53 R172H homolog) in human myoblasts promotes FAO. Myoblasts were transduced with empty vector control (-) or human p53 R175H cDNA lentivirus and used for the following assays. A, Palmitate-induced oxygen consumption rate (OCR) was measured by Seahorse with or without FAO inhibitor etomoxir (n =5). B, Immunoblots of enzymes involved in FAO. Note that CPT1B is specific to skeletal muscle tissue. C, Relative mRNA levels of the respective genes involved in FAO quantified by RT-PCR. p21 expression serves as a control for the dominant negative activity of the mutant p53 (n = 6). *P < 0.05.