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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Cancer Prev Res (Phila). 2020 Sep 21;14(1):31–40. doi: 10.1158/1940-6207.CAPR-20-0368

Figure 5.

Figure 5.

Haploinsufficiency of CPT2 in T cells decreases FAO and mTOR activity while increasing the survival time of p53172H/H mice. A, Kaplan-Meier survival plot of CD4-Cre+ p53172H/H mice with the indicated CPT2 genotypes. Statistical testing by log-rank (Mantel-Cox) test shows a significant difference between CPT2+/+ and CPT2+/fl genotypes. B, Spectra of cancer types diagnosed by necropsy and histopathology. C, Immunoblots of thymic lymphocytes from 10-wk old male mice of the indicated genotypes. D, In vitro fatty acid oxidation assay of thymic lymphocytes using 3H-palmitate as substrate (n = 3–4). Statistical difference by 1-way ANOVA. E, Heat map of metabolites identified by lipidomic profiling. Lipid species which were significantly different (P < 0.05) between the two genotypes are shown. Note the accumulation of long-chain free fatty acids in CPT2 deficiency. Metabolite ion abundance was normalized to the median and then log2 transformed. Color is based on the normalized ion abundance in the range of -1.0 to 0.6 (blue, lowest abundance; red, highest abundance) (n = 5). *P < 0.05, **P < 0.001.